New Human Papilloma Virus Isolated from Epidermodysplasia Verruciformis Lesions1

Human papilloma virus (HPV) was isolated from red plaques of a patient (N. F.) with epidermodysplasia verruciformis. Elec tron microscopic examination showed characteristic particles of papilloma virus as ¡cosahedrons about 45 nm in diameter. DNA was extracted from these particles, and closed-circular DNA (Form I) was purified by centrifugation in CsCI containing ethidium bromide. The molecular weight of the DNA was about 5.0 x 106. A physical map of the HPV DNA was constructed using several restriction enzymes. The restriction endonuclease cleavage pattern of the HPV DNA was different from those of other types of HPV reported thus far, suggesting that the isolate was a new, as yet unclassified, HPV.